Abstract
The metabolic controlling of tissue hydration is the fundamental parameter determining cell functional activity and its dysfunction is the common consequence of any cell pathology, including aging. The aim of the present study is to reveal the differences of age-dependent metabolic controlling of cell hydration of excitable tissue such as brain cortex and non-excitable tissues such as liver and spleen. For this purpose, the age-dependent sensitivity of cell hydration in excitable and non-excitablet issues is studied through depressing metabolic activity by cooling and its activation by supplying animals with distilled water, by inactivation of Na+/K+ pump and activation of Na+/Ca2+ exchange in the reverse mode. The obtained data bring us to the conclusion that the metabolic regulation of cell hydration in excitable tissue is realized by the activation of electrogenic Na+/K+ pump and Na+/Ca2+ exchange in the cell membrane and the formation of endogen water by mitochondrial activity, while the regulation of cell hydration in non-excitable tissue is carried out only by the activity of mitochondrial function. Aging leads to an over-expression of Na+/K+ pump units in liver and Na+/Ca2+ exchanger in brain cortex of rats.
Highlights
The aim of the present study is to reveal the differences of age-dependent metabolic controlling of cell hydration of excitable tissue such as brain cortex and non-excitable tissues such as liver and spleen
The obtained data bring us to the conclusion that the metabolic regulation of cell hydration in excitable tissue is realized by the activation of electrogenic Na+/K+ pump and Na+/Ca2+ exchange in the cell membrane and the formation of endogen water by mitochondrial activity, while the regulation of cell hydration in non-excitable tissue is carried out only by the activity of mitochondrial function
Aging leads to an over-expression of Na+/K+ pump units in liver and Na+/Ca2+ exchanger in brain cortex of rats
Summary
The dysfunction of metabolic controlling of cell hydration can be considered a common consequence of cell pathology, including aging. It is predicted that in the regulation of cell volume in ET cells involve the electrogenic ionic transporting mechanisms in the membrane and mitochondrial processes generating the water molecule release in cytoplasm. To check this hypothesis in the present work, the comparative study of the role of the membrane ion transporting mechanisms and the intracellular oxidative processes in age-dependent impairment of metabolic controlling of cell hydration in ET and NET in in vivo and in in vitro experiments are performed using different experimental conditions
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