Abstract
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Highlights
F February, 2009 lettucenin A is considered new at that time compared to others phytoalexins that found in soya bean, pea and many other plants
Silver nitrate elicited the highest accumulation of lettucenin A (4.27 μg/ml) but not significantly different with copper sulfate which induced the production of 4.26 μg/ml (Figure 1)
This followed by ultraviolet radiation contributed to 1.52 μg/ml of lettucenin A and freeze-thawed method induced the lowest production of lettucenin A with only 0.49 μg/ml (Figure 1)
Summary
Lettuce seeds were sown using top soil with mixture of compost to maintain the moisture of the soil. The lettuce seeds were first grown in trays before transferred to pots after two weeks old. Solution of AgNO3 was prepared at the concentration of 10 mM (1%). The lettuces were sprayed with solution of AgNO3 when they reached to their respective week before maintained for another three days (Mert-Turk et al 2003). Plants were placed in 25 ̊C under normal growing condition for three days before undergo extraction. Solution of 50 mM (5%) of CuSO4 was prepared and the whole plant was sprayed with the solution at the respective weeks (Al-Barwani and Eltayed 2004). Control plants were not sprayed with CuSO4. All plants were incubated for three days under the normal growing condition
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