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Abstract
A glucose sensor consisting of an enzyme membrane and an oxygen electrode was used to measure the glucose concentration in aqueous bovine serum hemoglobin solution. The enzyme membrane was an immobilized glucoxidase-PVC membrane having a finely porous structure.During application of the sensor to the solutions, bovine serum hemoglobin adsorbs on the surface of pores of the PVC membrane. Owing to the plugging of hemoglobin within the membrane, the steady-state output falls with time.Based on the reaction-diffusion model for the enzymatic reaction in the membrane, the diffusion coefficients and the reaction rate constant were evaluated. These parameters were successfully correlated with time. Calculated values of declining output of the sensor at steady state with time agreed well with the observed ones.
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