Abstract
A major problem still hindering the development of the free radical and the non-enzymatic glycation theories of aging is the fluorescence spectral discrepancies between measurements on lipofuscin/age pigment in situ (microfluorometry) and on extracted and dissolved materials (spectrofluorometry). In this study we have further investigated the concentration-dependent fluorescence-shift hypothesis suggested by Yin and Brunk (1). Malondialdehyde/glycine reaction products, acridine orange and quinine all showed pronounced concentration-dependent shifts of fluorescence excitation and emission maxima. The findings add further support to the concept that discrepant age pigment fluorescence data are due to variable quenching during measurements using different techniques.
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