Aggregation-induced emission enhancement of gold nanoclusters triggered by sodium heparin and its application in the detection of sodium heparin and alkaline amino acids

Abstract

This work first reported that sodium heparin could cause the aggregation-induced emission enhancement (AIEE) effect of GSH-AuNCs (Glutathione functionalized gold nanoclusters). While it was interestingly found that the addition of alkaline amino acids would greatly weaken this effect. Thus, fluorescent system was designed for the quantitative detection of sodium heparin and alkaline amino acids. Negatively charged sodium heparin would connect with GSH-AuNCs through electrostatic attraction, leading to a significant AIEE effect. Then alkaline amino acids would competitively bind with sodium heparin, causing this effect to almost disappear. The reasons were as follows: (I) The hydrogen bonding between sodium heparin and alkaline amino acids was much stronger than electrostatic force, causing GSH-AuNCs to be competitively replaced. (II) Alkaline amino acids and GSH-AuNCs were both positively charged and repelled each other. The presence of alkaline amino acids would hinder the AIEE effect. (III) AIEE effect was confirmed to have a close relationship with the pH value which could be greatly affected by alkaline amino acids. (Ⅳ) Not only the hydrogen bonding, the electrostatic force also existed between the alkaline amino acids and sodium heparin. All the above reasons worked together to weaken the AIEE effect of GSH-AuNCs triggered by sodium heparin. Finally, both sodium heparin and alkaline amino acids were accurately detected, showing good correlation coefficients of 0.99 with the LODs of 0.0100 mg/mL (sodium heparin), 1.05 μM (histidine), 3.38 μM (arginine) and 6.16 μM (lysine), respectively.