Abstract
Photodynamic therapy (PDT) is a noninvasive treatment for selectively killing malignant tumor cells. The photosensitizer is a necessary component of photodynamic nanomedicine. Many efforts have been made to develop new photosensitizers for efficient cancer photodynamic therapy. In this work, we report a novel nano photosensitizer, polymeric micelles (AIE-M) with aggregation induced emission characteristic, for photodynamic cancer therapy. AIE-M with sub-20 nm particle size is prepared by the self-assembly of salicylaldazine-incorporated amphiphilic polymer (AIE-1), which can produce reactive oxygen species (ROS) with light irradiation in solution. After uptake by cancer cells, AIE-M can specially sojourn in plasma membranes of cancer cells at the early stage and predominantly accumulate in the mitochondria of cancer cell at the late stage. The phototoxicity of AIE-M, resulting from the generation of intracellular ROS with light irradiation, can efficiently cause cancer cells death by apoptosis and necrosis. The advantages of AIE-M as a nano photosensitizer include the small size, highly colloidal stability in the process of preparation and storage, and high cell penetration. The ultra-low Critical Micelle Concentration (CMC) of AIE-1, negligible dark toxicity and super phototoxicity of AIE-M suggest its promising potential for image-guided PDT.
Highlights
Cancer has become a great threat to human health which is one of the main causes of human death, and a great burden on people’s psychology
Light with an appropriate wavelength, photosensitizers (PSs) and oxygen are the three important elements of photodynamic therapy (PDT), in which PSs are activated by light and transmit energy to oxygen to produce reactive oxygen species (ROS) including singlet oxygen (1O2), causing cancer cell apoptosis and necrosis [3]
HeLa cells were purchased from the China Center for Type Culture Collection (Wuhan University, Wuhan, China) and cultured in Dulbecco’s modified Eagle medium (DMEM) supplemented with 4 mM L-glutamine, 10% fetal bovine serum (FBS) and 1% antibiotics (100 U mL−1 penicillin and 100 mg mL−1 streptomycin) at 37 ◦C in a humidified atmosphere containing 5% CO2
Summary
Cancer has become a great threat to human health which is one of the main causes of human death, and a great burden on people’s psychology. Light with an appropriate wavelength, photosensitizers (PSs) and oxygen are the three important elements of PDT, in which PSs are activated by light and transmit energy to oxygen to produce reactive oxygen species (ROS) including singlet oxygen (1O2), causing cancer cell apoptosis and necrosis [3]. Upon light illumination, the intrinsic fluorescence of PSs is often detected and significant for imaging-guided therapy [4]. The development of PSs is one of the most important tasks in photodynamic therapy [5]. The encapsulation of many hydrophobic PSs into nanocarriers often resulted in aggregation-caused quenching (ACQ) in fluorescence and decrease of. The encapsulation of many hydrophobic oPfS2s0 into nanocarriers often resulted in aggregation-caused quenching (ACQ) in fluorescence and decrease of ROS production, which are common in the case of porphyrin and other traditional PSs. RThOeSdpirsocdouvcetriyono,fwahggicrhegaaretioconm-inmdounceind tehme icsassieonof(ApoIErp)hfyluroinroagnednostchaenr tpraedrfieticotlnyaloPvSesrc. KIn, wtheisrwepoorrkt, twhee ruepptoarktethoef uApIEta-kMe obfyAIHE-eMLabyceHlles,Laincterlalsc,eilnlutrlaarcelgluenlaerragteionneraotfionROofSROinSduincdeudcebdybyliglihgth, t,aanndd pphhoottooddyynnaammiicc iinnhhiibbiittiioonn ooff HHeeLLaa cceellllss ggrroowwtthh. HeLa cells were purchased from the China Center for Type Culture Collection (Wuhan University, Wuhan, China) and cultured in Dulbecco’s modified Eagle medium (DMEM) supplemented with 4 mM L-glutamine, 10% fetal bovine serum (FBS) and 1% antibiotics (100 U mL−1 penicillin and 100 mg mL−1 streptomycin) at 37 ◦C in a humidified atmosphere containing 5% CO2
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
