Abstract
Oligodeoxyribonucleotides (ODNs) that have four repeats of the human telomeric sequence d(TTAGGG) can assume several monomolecular G-quadruplex topologies depending on the cation species present, the bases at the 5' or 3' end, and on the sample preparation. The study of G-quadruplexes is important and biologically relevant due to their potential use as therapeutic targets. We have previously reported that under certain experimental conditions some quadruplex ODNs aggregate to form multi-molecular structures. These structures may be biologically relevant because of the similarity of their experimental conditions to in-vivo environments. We are interested in exploring the characteristics of the associated species and the nature of their stabilizing interactions. To this end we are investigating the effect of several parameters such as the sequence of the ODN, the concentration of the ODN, and the nature and concentration of the stabilizing cations. The ODNs under investigation are all derivatives of the human telomeric sequence. The equilibrium, kinetics, and structural properties of these structures are studied using circular dichroism spectroscopy (CD), UV spectroscopy, Raman spectroscopy, Dynamic light scattering (DLS) and analytical ultracentrifugation as a function of temperature and pressure.
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