Abstract
Concanavalin A (Con A) adsorbs on dipalmitoylphosphatidylcholine (DPPC):cholesterol bilayers but aggregation is only produced if neoglycolipids such as mannose–mannose–mannose-distearylglycerol (3M-diC18), glucose–mannose–glucose-distearylglycerol (GMG-diC18) or glucose–mannose–glucose-cholesterol (GMG-cho) are present. The phase state of the lipid, modified by the inclusion of cholesterol in the mixture, does not affect the aggregation of GMG-diC18:DPPC vesicles induced by lectin. With GMG-diC18 lipids, aggregation appears to be produced by the formation of a strong complex between the neoglycolipids and the protein. This interaction affects the hydrocarbon core of DPPC bilayers containing GMG-diC18. The greatest changes are observed when GMG-diC18 is present in the vesicle composition. The aggregation induced by Con A is maximal with 3M-diC18. In this case, the changes in surface properties and in the protein are less pronounced, evidencing an alternative mechanism of aggregation. The type of sugar unit and the lipid moiety by which neoglycolipids are anchored to the bilayer, determine the magnitude of the above mentioned processes.
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