Aggregation Behavior of Amyloid β1–42 Peptide Studied Using 55 MHz Wireless–Electrodeless Quartz Crystal Microbalance

Abstract

A homebuilt wireless–electrodeless high frequency quartz crystal microbalance is adopted for long-time monitoring of the aggregation behavior of amyloid β1–42 peptide in a flow-cell system. The monomer amyloid peptides are immobilized on both surfaces of the crystal, and an amyloid-β solution is injected. The monotonic frequency decrease indicates aggregation on the crystal, which yields aggregation rate. Aggregation is observed even at a peptide concentration as low as 550 nM.