Abstract
The 12S and 22S RNA fractions were isolated from phenol extract of the total polysome population of MOPC-321 mouse myeloma (a light-chain producer). Both RNA fractions exhibited mRNA activity when tested in the Krebs II ascites cell-free system. Fingerprint analyses of tryptic digests of the cell-free products showed that whereas the 12S RNA fraction contained L-chain mRNA together with a large amount of non-L-chain mRNAs, the 22S RNA fraction showed almost exclusively L-chain mRNA activity. The observed purification that was achieved by sucrose gradient centrifugation might be attributed to the tendency of L-chain to form aggregates upon exposure to phenol.
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