Abstract
Human semen serves as fluids medium in which sperm cells are dispersed and swim within. Semen contains about 90% water along with proteins that include Immunoglobulin-A (I g A), Haptoglobin, fructose sugar, other dissolved substances and electrolyte. Apart from preserving the sperm cells, semen may carry certain immunological feature as part of its protective functions. From a male partner in coital activity, semen’s cellular component and proteins deposited into the vagina do not antigenically qualify as ‘self’. This is a study to determine a possible agglutination resistant effect of semen on various types of ABO and Rhesus blood group members or agglutination enhancing effect of the same ABO and Rhesus group members. Keywords : Rhesus, ABO, Blood Group Agglutination Semen Haptoglobin. DOI: 10.7176/JMPB/71-01 Publication date: June 30 th 2021
Highlights
A prospective mother’s ovum carries antigens some of which are similar to that of the sperm cell from a destined biological father and others so different and this constitutes a lot of immunological challenges
Anti Sera from Biotect Laboratory were purchased to be the source of the various agglutinins, Forty test tubes were obtained for the work twenty of which are for the test group where where 0.5 ml of semen is incubated with 0.5 ml of various blood Rhesus +ve types of ABO system, while the other 20 are for the control group where no semen is added
Twenty mililitres of blood is obtained from each of the different donors with blood group A+, B+, AB+ and O+ and washed with normal saline as the source for the agglutinogens while the corresponding Anti Sera from Biotect Laboratory were purchased to be the source of the various agglutinins
Summary
A prospective mother’s ovum carries antigens some of which are similar to that of the sperm cell from a destined biological father and others so different and this constitutes a lot of immunological challenges. The acidic vaginal medium can be reduced through neutralization reaction by the alkaline nature of the semen as well as possible agglutination resistance between agglutinin(s) in vaginal secretion and agglutinogens from semen component(s). Anti Sera from Biotect Laboratory were purchased to be the source of the various agglutinins, Forty test tubes were obtained for the work twenty of which are for the test group where where 0.5 ml of semen is incubated with 0.5 ml of various blood Rhesus +ve types of ABO system, while the other 20 are for the control group where no semen is added. Twenty mililitres of blood is obtained from each of the different donors with blood group A+, B+, AB+ and O+ and washed with normal saline as the source for the agglutinogens while the corresponding Anti Sera from Biotect Laboratory were purchased to be the source of the various agglutinins. Agglutinin reaction is observed with both the unaided eye and under microscopy as formation of clump of particles
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