Abstract
Antiglobulins to vegetative cells and to intact spores of the bacteria Bacillus popilliae and Bacillus lentimorbus were used to study the antigenic relationship of milky disease bacteria with other sporeforming bacilli. Vegetative cells from 12-hr cultures were used as test antigens. The species of milky disease organisms were serologically distinct with neither vegetative cells nor spores exhibiting cross reactivity. However, B. popilliae was related to several other sporeforming insect pathogens, both those with swollen sporangia and those with crystalliferous inclusions. Bacillus larvae was serologically related to both organisms. Of the Bacillus species nonpathogenic for insects, no strain tested agglutinated with any of the antiglobulins to the milky disease bacteria.
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