Agglutinability of cattle red cells. 2. Agglutination by isologous and heterologous sera of enzyme-treated red cells.

Abstract

SummaryNeuraminidase treatment made cattle red cells (CRC) agglutinable by the agglutinins of the different heterologous but not of the homologous sera. These agglutinins were, however, absorbed by both the nauraminidase‐treated and the intact CRC.Proteolytic treatment made CRC agglutinable also by the normal cattle, isoimmune and autologous sera. Agglutination titres of the CRC ranged from 1: 2 to 1: 256, but the variation between CRC from members of monozygous (MZ) pairs was not greater than ± 2 agglutination score units the range of experimental error.Treatment with trypsin made the A1, A2 factors more emergent on the surface of CRC for agglutination by anti‐A2, while pronase treatment had a similar effect upon agglutination of Z‐positive cells by anti‐Z.