Age-related examination of gluco-, and mineralocorticoid effects in vasopressin deficient Brattleboro rats

Abstract

Event Abstract Back to Event Age-related examination of gluco-, and mineralocorticoid effects in vasopressin deficient Brattleboro rats János Varga1*, Szilamér Ferenczi2, Krisztina Kovács2, Daniela Jezova3 and Dóra Zelena1 1 Institute of Experimental Medicine, Department of Behavioral Neurobiology, Hungary 2 Institute of Experimental Medicine, Laboratory of Molecular Neuroendocrinology, Hungary 3 Slovak Academy of Sciences, Institute of Experimental Endocrinology, Slovakia Previously we proved that vasopressin takes place an age-related manner in the regulation of the hormone levels of the hypothalamo-pituitary-adrenal axis. The effector molecules of the axis are glucocorticoids, which are structurally very similar to mineralocorticoids, and they can act on the same receptors. Therefore the aim of our present work was to examine the age-related influence of vasopressin on gluco- and mineralocorticoid levels and their receptors. Perinatal (10 days old) and adult (10 weeks old) male rats were used. We compared vasopressin deficient Brattleboro rats to their heterozygote littermates. Corticosterone (glucocorticoid) and aldosterone (mineralocorticoid) levels were measured by radioimmunassay under resting conditions and after hypoglycemic stress. Gluco- (GR) and mineralocorticoid receptor (MR) mRNA levels were measrured by quantitative PCR in the hypothalamus and liver. Independetly from the genotype the smaller rats showed only minimal corticosterone increase to stress, while their aldosterone elevation was more expressed compared both to adults and to corticosterone levels. The level of GR and MR mRNA were smaller in the hypothalamus and liver of 10 days old rats. In 10 days old vasopressin deficient rats the corticosterone levels were higher, while their GR and MR mRNA levels were lower in the liver (weeker effect) and higher in the hypothalamus (stronger feedback) compared to heterozygous animals. Our results supports the existence of stress hyporesponsive period, while 10 days old rats showed smaller glucocorticoid elevations with lower receptor levels compared to adult. The opposite (stress hyperresposibility) was true for the pups' aldosterone levels. In general the consequences of vasopressin deficiency was more pronounced in 10 days old animals supporting the view that vasopressin plays a more considerable regulatory role in the perinatal age. Conference: IBRO International Workshop 2010, Pécs, Hungary, 21 Jan - 23 Jan, 2010. Presentation Type: Poster Presentation Topic: Homeostatic and neuroendocrine systems Citation: Varga J, Ferenczi S, Kovács K, Jezova D and Zelena D (2010). Age-related examination of gluco-, and mineralocorticoid effects in vasopressin deficient Brattleboro rats. Front. Neurosci. Conference Abstract: IBRO International Workshop 2010. doi: 10.3389/conf.fnins.2010.10.00136 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 28 Apr 2010; Published Online: 28 Apr 2010. * Correspondence: János Varga, Institute of Experimental Medicine, Department of Behavioral Neurobiology, Budapest, Hungary, inajajani@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers János Varga Szilamér Ferenczi Krisztina Kovács Daniela Jezova Dóra Zelena Google János Varga Szilamér Ferenczi Krisztina Kovács Daniela Jezova Dóra Zelena Google Scholar János Varga Szilamér Ferenczi Krisztina Kovács Daniela Jezova Dóra Zelena PubMed János Varga Szilamér Ferenczi Krisztina Kovács Daniela Jezova Dóra Zelena Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.