Age-related change of neuropeptide Y-immunoreactive neurons in the cerebral cortex of aged rats

Abstract

Recent studies have explored certain changes with aging of neurons containing neuropeptides. The extent of loss in aged central nervous system (CNS) of neuronal cells containing neuropeptide Y (NPY) has not yet been established with certainty, and available data is often contradictory. Changes of NPY-containing neurons with aging in the cerebral cortex of aged rat were demonstrated by immunocytochemistry. A major loss of NPY-immunoreactive (ir) neurons in the aged rat brain was observed in the retrosplenial cortex, frontal cortex area 1 and 2, parietal cortex area 1 and 2, occipital cortex area 1 and 2, temporal cortex area 3, cingulate cortex and the hippocampus proper. A loss of NPY-ir neurons was observed mostly in layers V and VI; in addition, the number and length of dendritic branches appeared to be decreased and shortened in the aged group. These results indicate the involvement of NPY-ir neurons in the aging process of cerebral cortex, and provide the first morphological evidence for the loss of NPY neurons in the cerebral cortex of aged rats.