Abstract
T. durum wheat seeds (caryopses) were stored for 18 months in sealed containers containing gaseous CO 2 under pressure. The seeds were then stored for 16 years in air under laboratory conditions. Germination capacity of the seeds was evaluated at 2-year intervals and when the old seeds displayed a very low germination power, their capacity to produce in vitro callus and plantlets, starting from the 1st node tissue of the embryos, was evaluated. The results show that early CO 2 treatment of seeds reduces the negative effect of ageing. The CO 2 treated seeds maintained viability for longer periods and the embryo tissues explants gave calli and produced plantlets whereas CO 2 untreated seeds did not. The reduction of oxidative processes during early storage periods and the usefulness of CO 2 treatments for prolonging seed life-span are considered.
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