Abstract
Recent decade has seen a significant increase in the average age at which couples first produce, thus it becomes essential to study and understand how age affects fertility in humans. Age-dependent variations in human seminal plasma proteome were identified by two- dimensional differential gel electrophoresis (2D-DiGE) followed by mass spectrometric analysis. Overall, 10 differentially expressed proteins were identified, viz. glutaredoxin domain-containing cysteine-rich protein-2, clusterin, serum albumin, translation initiation factor IF-2 like, alternative protein RRT-34, ecto-ADP-ribosyltransferase 4, protein Unc-119 homolog A, CB1 cannabinoid receptor-interacting protein 1 and serotransferrin. Expression of clusterin was found upregulated in older age samples, which was further validated by western blotting analysis. It is evident from available literature that higher expression of clusterin is associated with poor semen quality. Based on this, our study for some extent concludes and supports the fact that semen quality declines with increasing age.
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