Abstract
BackgroundGut bacteria are an important component of the microbiota ecosystem in humans and other animals, and they play important roles in human health. The aim of this study was to investigate the relationship between gut microbiota and multiple demographical-, behavioral-, or biochemical-related factors in subjects with chronic disease. Subjects with a very wide age range who participated in community-based chronic disease prevention and screening programs in China were enrolled. We analyzed the intestinal microbiota composition using 16S rRNA-based high-throughput sequencing of fecal samples, analyzed the association between gut microbiota structure and multiple demographical, behavioral, and biochemical factors, and compared the differences in microbiota composition in age-stratified groups with different blood glucose levels.ResultsOur results showed that both age and blood glucose levels had a significant impact on the gut microbiota structure. We also identified several taxa showed distinct abundance in groups with different glucose levels. Lactobacillus and Bifidobacterium at genus level and their related taxa were more abundant in the GLU high group comparing with GLU normal group and in NGR group comparing with DM group. Further analysis using the age-stratified data showed that blood glucose levels had a more significant impact on the gut microbiota in the ≥76 y age group than in the ≤75 y age group, which indicated that it is necessary to take age into account when conducting such studies. Moreover, we identified several taxa that were highly associated with blood glucose levels in the ≥76 y age group but not in the ≤75 y age group. Within the ≥76 y age group, Lachnospiraceae incertae sedis and Bacteroides were more abundant in the GLU normal group, whereas Lactobacillus and Bifidobacterium at genus level were more abundant in the GLU high group.ConclusionsThis result suggested that taxa that are capable of differentiating blood glucose levels might differ significantly in different age groups.
Highlights
Gut bacteria are an important component of the microbiota ecosystem in humans and other animals, and they play important roles in human health
We investigated the relationship between gut microbiota and plasma glucose levels, age, as well as multiple demographical, behavioral, and biochemical-related factors in subjects with a very wide age range who participated in community-based chronic disease prevention and screening programs in China
Among the 55 participants who were included in the high blood glucose group (HGR), 22 had fasting plasma glucose level between 6.1 and 7.0 and were categorized as the impaired fasting glucose group (IFG), while 33 had fasting plasma glucose level no less than 7.0 and were categorized as the diabetic group (DM)
Summary
Gut bacteria are an important component of the microbiota ecosystem in humans and other animals, and they play important roles in human health. The aim of this study was to investigate the relationship between gut microbiota and multiple demographical-, behavioral-, or biochemical-related factors in subjects with chronic disease. We analyzed the intestinal microbiota composition using 16S rRNAbased high-throughput sequencing of fecal samples, analyzed the association between gut microbiota structure and multiple demographical, behavioral, and biochemical factors, and compared the differences in microbiota composition in age-stratified groups with different blood glucose levels. Gut bacteria are an important component of the microbiota ecosystem in humans and other animals, and they play important roles in human health, such as nutrient absorption, homeostatic control of energy balance, immunoregulation, gastrointestinal development, and many other physiological processes.
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