Age‐related oxidation of lipids and proteins in rats' crystalline lens

Abstract

AbstractPurpose estimation and comparison of age‐related intensity of oxidation of lipids and proteins of rats' crystalline lensMethods Experiments are carried out on 25 Wistar line rats males of four age groups: 5 (n=6), 12 (n=7), 24 (n=6) and 36 (n=6) of months. Homogenate of crystalline lens tissue was used. The level of the general lipids, the contents of diene and triene conjugates, level of the Schiff bases and TBA‐test were estimated. The received results were rated to amount of lipids and expressed in relative units. Extent of oxidizing proteins modification, the content of the general protein was defined. The level of oxidizing proteins modification was rated to amount of proteins. Potential possibility of oxidation was estimated by method of the induced chemiluminescence with use of Fenton's reactionResults In adult animals' crystalline lenses (12 months) the raised level of products of lipids oxidation was observed. Then, the level of trienic conjugates were decreasing in the interval of 12‐24 months. The comparative content of carbonyl derivative of proteins had tendency to decrease with age. At groups of age 12, 24 and 36 months in comparison with group of 5 months were determined the decrease of the hemiluminescence level. It corresponds to decrease of level of the medium for free radical oxidationConclusion On the basis of results, we can assume that, together with the intermolecular interaction of proteins with each other and with low‐molecular metabolites, the free‐radical processes of lipids proceeding on membranes, also contributes to formation of age‐related changes of lens in rats