Abstract
The increasing incidence of type 1 diabetes coupled with advances in treatment of type 1 diabetes has resulted in an unprecedented number of older adults living with and controllable type 1 diabetes. The objective of this experimental study was to assess the impact of aging on the level of basal glycaemia and activities of glucose-6-phosphate dehydrogenase [EC1.1.1.49], pyruvate kinase [EC 2.7.1.40] and glutathione reductase [EC1.6.4.2] in erythrocytes of alloxan-diabetic rats. Methods: We used 100 male Wistar rats, divided into two age groups: I group included- 2-month (adult) animals, and II group was made up of 4-month (old) animals. Diabetes was modelled by injecting the rats with 5% solution of alloxan monohydrate intraperitoneally in a dose of 170 mg/kg. Blood was taken from the tail vein to evaluate the basal glycaemia on 5-th and 47-th day after the alloxan injection. Rats were sacrificed on the 47-th day of the experiment in accordance with the regulations on ethical treatment of vertebrates. The assessment of the activity of the enzymes was carried out by standard methods. Statistical analysis was performed by using Statistica 10 StatSoft Inc. Results. The level of basal glycaemia on the fifth day of the experiment in the animals of both groups went up on average by 115% from baseline values. We founded that on 47-th day this index was higher in group of old rats by 20% than in adult rats. Pyruvate kinase activity in erythrocytes of adult and old animals with diabetes decreased by 35% and 50% respectively compared with the control. Glucose-6-phosphate dehydrogenase activity in erythrocytes of adult and old animals with diabetes decreased by 27% and 45% respectively compared with the control on 47-th day. The changes may be considered as the result of age-related disorders of glucose metabolism due to disturbances in free radical mechanisms. Glutathione reductase activity in erythrocytes of adult and old animals with diabetes decreased by 29% and 35% respectively compared with the control on 47-th day. Conclusion. We have determined when getting aged, the alloxan-diabetic rats demonstrate changes in the sensitivity of pyruvate kinase, glucose-6-phosphate dehydrogenase and glutathione reductase activities in erythrocytes resulted from the effect of diabetes mellitus factors (hyperglycaemia). We can suggest that glycaemic control is key purpose for older patients with type 1 diabetes in order to prevent of complication, which can be aggravated with age.
Highlights
Diabetes mellitus (DM) is one of the most prevalent endocrine diseases throughout the world
The study was carries out in compliance with the Rules for All Vertebrate Animal Studies (1977) and the European Council Convention for the Protection of Vertebrate Animals used in experiments and for other scientific purposes (Strasbourg, 1986), and according to the directions of International Committee of Medical Journals Editors (ICMJE), as well as to Bioethical expertise of preclinical and other scientific researches conducted on animals (Kyiv, 2006).We used 100 male Wistar rats divided into two age groups: the I group included 2-month rats, and the II group included 4-month rats
Control DM in adult rats DM in old rats atic share reliably decreased by 55%, number and percentage of beta-cells with necrosis decreased diminished by 90% and 97% respectively compared
Summary
Diabetes mellitus (DM) is one of the most prevalent endocrine diseases throughout the world. It was revealed that the median of CD44+ epitheliocytes area in polyps two-fold smaller than the median of CD44+ cancer cells area in colorectal adenocarcinoma: 15,67 (12,47 ; 19,47) % vs 30,60 (24,56 ; 36,45 ) % , р 0,05. Distal colonic polyps are characterized by membranous CD44 expression with the median of CD44+ epitheliocytes area that is two-fold smaller than the median of CD44+ cancer cells area. Colorectal adenocarcinoma is characterized by membranous-cytoplasmic CD44 expression with the median of CD44+ stromal cells area that significantly increases during the tumor progression from I to III stages and with the median of CD44+ cancer cells area that significantly go up during the tumor progression from III to IV stages
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