Age-related changes in the gut melatonin levels and its possible role in the regulation of feeding and digestibility, with the development of the gut from fingerling to adult stages of carp, Catla catla.

Abstract

The present study aims to understand the feeding-related age-bound changes in gut histoarchitecture and its response to gut melatonin (GM) titer regulating major digestive enzymes in carp,Catla catla. Therefore, gut samples were collected from different growth stages of carp, viz. (i) fingerling (FL), body weight (BW) ≥ 3g to ≤ 20g; (ii) advanced fingerling (AFL), BW > 20g to ≤ 40g; (iii) early juvenile (EJv), BW > 40g to ≤ 70g; (iv) juvenile (Jv), BW > 70g to ≤ 200g; (v) late juvenile (LJv), BW > 200g to ≤ 300g; (vi) preadult (PA), BW > 300g to ≤ 500g; (vii) subadult (SA), BW > 500g to ≤ 1.00kg; and (viii) adult (AD), BW > 1kg to ≤ 2.5kg. Data analysis revealed that the highest titer of GM was noted in FL, moderate in AFL, Jv, and PA, lower in EJv, SA, and AD, and lowest in LJv. Results depicted a negative correlation between the development of the gut and its melatonin content. Moreover, GM was positively associated with feeding intensity and gastro-somatic index (GaSI) and negatively related to ovarian onset and development. Following correlation and principal component analysis, several pieces of evidence were recorded on the role of gut melatonin in regulating digestive physiology. Finally, it indicates that gut melatonin has a progressively influential role in improving digestion, particularly protein and microbial digestion, with the development of an adult gut from the fingerling stage.