Age-Related Changes in the Blood Digestive Enzyme Amylase in Pheasants (Phasianus Versicolor)) In Ontogenesis

Abstract

The work presents age-related data on changes in the digestive enzymes of the blood in pheasants in ontogeny. The research is devoted to the artificial breeding of one of the subspecies of pheasants of our wild fauna (the emerald pheasant - Phasianus versicolor). The aim of the work was to determine the digestive enzyme amylasecontent in the blood plasma of pheasants in post-embryogenesis in comparison with the indicators in the chicks of the original line when using a complex of vitamins and anti-stress drugs. As a result of experimental studies, a change in the blood plasma digestive enzyme amylaseof pheasants from the moment of hatching and up to 2 months of age was revealed, with the complex use of vitamins (A-20000 IU, D3 -1250 IU, E-50 mg), as well as anti-stress preparations of succinic acid at a dose of 0.02 g, 0.03 g, 0.05 g per 1 kg of body weight per day. The determination of activity of the digestive enzyme amylase of the blood was carried out spectrophotometrically according to the method of Karavea, Spectrophotometer Specol 1500 (Analitik Jena), at a wavelength of 690 nm. Amylase activity indices in 1, 5, 10, 20, 30, and 60-day-old pheasants correspond to 517.8 ± 12.3, 402.5 ± 9.6, 273.1 ± 10.5, 161.6 ± 12.8, 254.5 ± 23.4, 210.7 ± 14.3 U/l. It was found that the activity of the enzyme in 1-day-old pheasants significantly exceeds the activity of amylase in 5, 10, 20, 30 and 60-day-old pheasants by 1.3, 1.9, 3.2, 2.0, 2.5 times, respectively.