Abstract
To investigate the effect of the culture age on Penicillium erythromellis cell wall polysaccharides, 7- to 59-day-old cell wall material was prepared and its chemical composition analysed. The yield of cell wall material increased throughout the incubation time, reaching up to 3.8 g/1 (59 days). Cell walls were mainly composed of neutral sugars (35–43%) and chitin (27–38%). The neutral sugars identified and quantitified by gas-liquid chromatography were mannose, galactose and glucose. Mannose and galactose content increased slightly during the incubation period (from 2.8 to 4.7 and from 100 to 11.3%, respectively). Glucose increased up to 29.7% (31 days) and then remained constant. Chitin content was maximum at the beginning and end of the incubation period. Sequential fractionation of 18-day-old cell wall material with alkali and acid indicated that it consisted of alkali-soluble polysaccharides containing glucose, galactose and mannose, and an alkali-insoluble β-glucan chitin complex containing xylose.
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