Age-related changes in NG2-expressing telocytes of rat stomach.

Yasuhisa Tamura,Kumi Takata,Asami Eguchi,Mitsuyo Maeda,Yosky Kataoka,Atsushi Asakura

doi:10.1371/journal.pone.0249729

Abstract

NG2 immunoreactive cells (NG2 cells) are found in the brain and peripheral tissues including the skin, intestinal tracts, and bladder. In a previous study, we observed the presence of NG2 cells in the stomach using bioluminescence imaging techniques in NG2-firefly luciferase (fLuc) transgenic (Tg) rats. Here, we aimed to identify and characterize NG2 cells in the adult rat stomach. Immunohistochemical studies showed that NG2 cells were mainly present in the lamina propria and most of the cells were gastric telocytes, co-expressing CD34, and platelet-derived growth factor receptor alpha (PDGFRα), with a small oval-shaped cell body and extremely long and thin cellular prolongations. In the rat stomach, NG2-expressing telocytes comprised two subpopulations: NG2+/CD34+/PDGFRα+ and NG2+/CD34+/PDGFRα-. Furthermore, we showed that the expression of NG2 gene in the aged rat stomach decreased relative to that of the young rat stomach and the decline of NG2 expression in aged rats was mainly observed in NG2+/CD34+/PDGFRα+ telocytes. These findings suggested age-related alterations in NG2+/CD34+/PDGFRα+ telocytes of rat stomach.

Highlights

To confirm whether PDGFRβ-expressing cells are pericytes based on their location, double immunostaining was performed with PDGFRβ and CD31 antibodies
These findings suggest that NG2+/CD34+/PDGFRα + cells and NG2+/CD34+/PDGFRα- cells in the rat stomach correspond to PDGFRαhigh telocytes and PDGFRαlow stromal cells, respectively
Immunostaining studies showed that some NG2 cells were immunoreactive for PDGFRβ, a marker of pericytes (S2B Fig) and that PDGFRβ+ cells were located near CD31-expressing endothelial cells (S2C Fig)

Summary

Objectives

We aimed to identify and characterize NG2 cells in the adult rat stomach. The aim of this study was to examine the localization and characterization of NG2 cells in the rat stomach and to investigate changes of NG2 cells in aged rat stomach.

Methods

Results

Conclusion