Abstract
Fluorescent material consisting of Schiff-base products increased with age in the chloroform-methanol extracts of the adult housefly, Musca domestica. The autofluorescent granules observed in tissue sections are identical to lipofuscin. The fluorescent spectra of the tissue extracts had an excitation maximum of 365 nm and an emission maximum of 435 nm. The fluorescent intensity of Eu (III) added to the chloroform-methanol extracts increased due to the energy transfer from the Schiff-base products. The highest concentration of the fluorescent substances occured in the head, however, the autofluorescent granules observed by fluorescence microscopy were most numerous in the abdominal tissues.
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