Abstract
This research measured lipid peroxidation products in rats of varying age by a new method. Wistar male rats, 4, 12, 22 and 32 months old, were examined for lipid peroxidation in vivo by measurement of ethane, ethylene, butane and pentane in breath gases of intact animals. In the older rats, the amounts of the four hydrocarbons exhaled were greater than those in younger rats. All hydrocarbons tested were related to age by an exponential relationship, and quantitatively, ethane and ethylene were related to age with a linear regression fit correlation coefficient, 0.466–0.622 (p<0.01-0.001). When hydrocarbon gas production of 32 month-old rats was compared with that of 4 month-old rats, the greatest ratio was that for pentane (1.99). The following order was ethylene >ethane>butane. There were significant differences in the production of all hydrocarbons between 32 month-old rats and 4 month-old rats. Thiobarbituric acid reactants in serum exhibited an increasing tendency with age, but the values of 32 month-old rats were lower than those of 22 month-old rats. However, the differences between the age groups were not significant. These results showed that the measurement of hydrocarbons in the rats'breath was a sensitive index of in vivo peroxidation during aging.
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