Age-related biochemical and histological changes in the soleus muscle of male mice

Amira R Hammad,Samaa M Bakr,Khairat B El-Baradie,Abeer A Alm-Eldeen

doi:10.21608/djs.2020.139233

Amira R Hammad, Samaa M Bakr + Show 2 more

Open Access

https://doi.org/10.21608/djs.2020.139233

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Abstract

During aging, skeletal muscle is subjected to progressiveloss of muscle mass and strength (sarcopenia) and a decline in thefunctional ability. The reduction in the antioxidative capacity ofthe skeletal muscle resulting in an abnormal accumulation of thereactive oxygen species (ROS) that is one of the possible causesof the decline in muscle performance. In this study thehistological changes in the soleus muscles of male mice at the ageof 1st and 15th old were examined and the activities of theantioxidant enzymes, CAT and SOD, non- enzymatic antioxidantGPH and MDA level were measured to clarify the age-relatedchanges in healthy subjects. Results showed that there is asignificant reduction (P ≤ 0.05) in the antioxidants SOD, CATand GPH and there is a significant increase (P ≤ 0.05) in MDAlevel at the age of 15th month when compared to 1st month old.We concluded that the activities of the antioxidants were affectedby aging. Moreover, there is age- related lipid peroxidation.

Highlights

Aging is characterized by a decrease in the physical function of the daily activities resulting in reduced quality of life
The present study was designed to investigate the age related biochemical and histological changes occurring in the skeletal muscles of the male mice
The current results showed a significant decrease in the activities of GSH, superoxide dismutase (SOD) and CAT, and a significant increase in MDA level with aging

Summary

Introduction

Aging is characterized by a decrease in the physical function of the daily activities resulting in reduced quality of life. Sarcopenia is one of the main characteristics of aging and, is characterized by loss of skeletal muscle mass and strength (Edstrom et al, 2007; Frontera and Ochala, 2015; Musumeci et al, 2015). The muscle reduced in size progressively, and muscle fibers were replaced by fat and fibrous tissue. This causes an increase in the oxidative stress, changes in the muscle metabolism, and degeneration of the neuromuscular junction, resulting in progressive loss of muscle function and frailty (Tsochatzis et al, 2014). Sarcopenia increased by 14% in people those aged between 65 and 70 years and 53% after 80 years (Santilli et al, 2014)

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