Abstract
In order to investigate the effects of age on androgen receptor (AR) in testes and epididymides of mandarin voles（Lasiopodomys mandarinus）, the expression of AR from five age groups [postnatal 1(neonatal), 10, 25, 45 and 60 days (adult) of age] was examined using immunohistochemistry method. The results were as follows: ① There was AR expression in leydig cells in neonatal voles and the expression of AR decreased at postnatal 10 days and 25days. AR expression reached its peak at postnatal 45 days and then decreased in adults（P＜0.05）. ② The positive expression of AR in myoid cells was found from neonatal to adult. The positive expression of AR was unchanged at postnatal 1 day and postnatal 10 days, but decreased at postnatal 25 days, reaching its highest level at postnatal 45 days and then decreased in adults (P＜0.05). ③ The positive expression of AR in prospermatogonia was weak in neonatal voles. There was no expression of AR in spermatogonia at postnatal 10 days. There was AR expression in spermatoon at postnatal 25 days, while in spermatocyte and some spermatoon at postnatal 45days. The positive expression of AR in spermatogonia, spermatoon and sperm was also found in adults. ④ The positive expression of AR in sertoli cells was found in adults, but there were few expressions of ARs at other ages. ⑤ The positive cells of ARs were both detected in epithelium cells and stroma cells of epididymides. These results suggest that AR expressiones in leydig cells, myoid cells and spermatogenic cells change significantly with mandarin vole’s individual development. Androgen facilitates leydig cells differentiation during puberty, and myoid cells play an important role during spermatogenesis. Androgen may regulate function of epididymises.
Highlights
In order to investigate the effects of age on androgen receptor (AR) in testes and epididymides of mandarin voles(Lasiopodomys mandarinus), the expression of AR from five age groups [postnatal 1(neonatal), 10, 25, 45 and 60 days of age] was examined using immunohistochemistry method
3 The positive expression of AR in prospermatogonia was weak in neonatal voles
5 The positive cells of ARs were both detected in epithelium cells and stroma cells of epididymides
Summary
Age Dependent Expressiones of Androgen Receptores in Testes and Epididymes of Mandarin Voles(Lasiopodomys mandarinus) Abstract: In order to investigate the effects of age on androgen receptor (AR) in testes and epididymides of mandarin voles(Lasiopodomys mandarinus), the expression of AR from five age groups [postnatal 1(neonatal), 10, 25, 45 and 60 days (adult) of age] was examined using immunohistochemistry method. 2 The positive expression of AR in myoid cells was found from neonatal to adult. 附睾内精子的成熟、获能都有非常重要的作用。雄 激素通过与雄激素受体(androgen receptor, AR)结 合发挥生理作用。有研究发现,AR基因突变或缺失 会导致雄性生殖器官发育畸形甚至不育 (Brinkmann et al,1995)。对大鼠、小鼠和成人研 究表明睾丸生精细胞(spermatogenic cells)内没有 AR(Bremner et al,1994;Zhu et al,2000;Carlos et al,1999;Zhou et al,2002)。但也有研究发现睾 丸组织内 的 精原细胞(spermatogonia)、精母细胞 (spermatocyte)、精子细胞(spermatoon)有AR阳性表 达(Kimura et al,1993;Vornberger et al,1994)。 从14天的小鼠胚胎至生后56天的小鼠睾丸,前精原 细 胞 ( prospermatogonia ) 与精原细胞有 AR 表 达 (Zhou et al,1996)。刚出生大鼠的前精原细胞内 有AR表达;生后2周龄,精子细胞开始出现AR表达; 生后1月龄,精原细胞开始出现AR表达;生后2月龄 精子出现AR表达;生后25月龄未见AR表达的生精 细胞(Wang et al,2003)。这些实验说明生精细胞 内AR的存在与否与实验动物的年龄(发育阶段)有 关。但这些研究大多集中于大鼠、小鼠、家畜(Kotula et al,2000)或人,对野生物种的研究较少,那么 野生种睾丸的发育过程中,AR在生精细胞内的表达 是否与实验动物一样呢?
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