Abstract
Aims This study examines the effect of chronic ouabain-treatment on renal Na + handling in 12-week and 52-week old rats. Main methods Wistar Kyoto rats aged 5 weeks or 45 weeks were treated with ouabain or vehicle during 7 weeks. Blood pressure was measured in conscious animals throughout the study. After 7 weeks of treatment urinary electrolyte concentration, Na +,K +-ATPase activity and α 1-subunit expression were determined in 12-week and 52-week old rats. Key findings In 12-week and 52-week old rats ouabain produced a significant increase in systolic blood pressure. Although no differences were observed in Na + excretion in these animals, 12-week old ouabain-treated rats had lower Na +,K +-ATPase activity in proximal tubules. However, 12-week old ouabain-treated rats had decreased fractional excretion of Na +. In proximal tubules of 52-week old rats Na +,K +-ATPase activity did not differ between vehicle and ouabain-treated groups. Significance Our results show that in Wistar Kyoto rats renal response to ouabain treatment may be age-dependent and that the hypertensive effect of ouabain is independent of the effect on renal Na +,K +-ATPase.
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