Abstract
We previously demonstrated that the hypothalamic supraoptic nucleus (SON) undergoes a robust axonal sprouting response following unilateral transection of the hypothalamo‐neurohypophysial tract in a young, 35‐day‐old rat. However, no collateral sprouting occurs following axotomy in a 125‐day‐old rat. Our lab has in vitro evidence demonstrating the role of CNTF (ciliary neurotrophic factor) in promoting process outgrowth via PI3K signaling. Thus, we compared the protein levels of the PI3K catalytic and regulatory subunits in the SON between 35‐ and 125‐day rats. We found less protein of the catalytic domain, PI3K p110γ, in the 125‐day rat SON; however, we did not observe changes in the other three catalytic domains nor the regulatory domains that we analyzed. Dual fluorescent studies demonstrated that all PI3K catalytic and regulatory subunits analyzed are localized to astrocytes and neurons in the SON. These data suggest that the lack of PI3K p110γ found in the 125‐day rat SON may prevent the collateral sprouting response from occurring, but future studies need to be performed to confirm this hypothesis.Support or Funding InformationThis material is based upon work supported by the National Science Foundation under Grant No. 1643998
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