Age-dependent changes of protein structure: The Properties of Young and Old Rabbit Aldolase Are Restored After Reversible Denaturation

Abstract

The significantly increased helical content is observed in muscle aldolase molecule of old rabbits. The unfolding and refolding of protein conformation followed by circular dichroism, fluorescence and enzyme activity showed the recovery of initial conformation after the denaturation. The protein folds into that existed prior to denaturation — “young” into “young” and “old” into “old” — the conformational differences between them being restored. This suggests that the primary structure modifications prior to the folding of the native protein conformation are the origin of the age-dependent differences of aldolase structure and function.