Age-dependent changes in liver ganglioside levels.

Abstract

Gangliosides, sialic acid-containing glycosphingolipids, are found in the outer layer of the plasma membrane of all vertebrate tissue cells; the highest concentration is in the central nervous system. In recent years, there has been research on the distribution and quantity of gangliosides in extra-neuronal tissues, such as liver, kidney and intestine. Since liver is the main source of gangliosides that are carried by lipoproteins in the blood, we examined the effect of development and aging on gangliosides in liver tissue. The relationship was investigated between GM1, GD3, GD1a, GD1b, GT1b ganglioside fractions and the aging process in liver tissue of Wistar-Albino rats aged 3, 6, 12 and 24 months. HPLC analysis of liver gangliosides showed the following results: Compared to 3 month-old rats, the GM1 fraction was decreased by 50% in 6 month-old rats, increased in 12 month-old rats and decreased in 24 month-old rats. The GD3 and GD1b fractions increased until 12 months of age and were decreased significantly (p < 0.01) in 24 month-old rats. The GD1a ganglioside fraction was significantly increased in 6 and 24 month-old rats (p < 0.01). We concluded that the increment of the polar fractions, such as GD3 and GD1b, and the variations of the other fractions in the plasma membrane of the hydrophilic liver tissue during the first 12 months were important parameters.