Abstract

We have compared the variation in histamine content of the venom of worker honey bees maintained in the field with those in an indoor flight room. We find that the temporal changes in venom histamine content are parallel under the different conditions. We have measured the histamine content of different regions of the venom system of virgin queen bees and found temporal changes in histamine concentration which parallel those seen in workers. While the absolute amounts of histamine in the queen venom system exceed those measured in workers, we estimate that the histamine concentration in queen and worker venom is similar. The LD 50 of histamine, injected into the haemocoele of worker bees in saline, is found to be 3.2 μg/worker.

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