Abstract
BackgroundTP53 is an attractive candidate for modifying age of onset (AO) in Huntington disease (HD): The amino-terminus of the mutated huntingtin (htt) exon 1 translation product has functional properties which may affect critically the TP53 pathway in HD neurons. The pathogenic domain of mutant htt interacts with nuclear transcription factors, and it potentially modulates TP53-induced transcriptional events. A single nucleotide polymorphism (SNP) resulting in the R72P exchange in TP53 protein might modulate the variation in AO. In addition, also the R196K replacement in human caspase activated DNase (hCAD) may theoretically affect the AO.MethodsWe have genotyped the polymorphisms R72P and R196K in a well established cohort of 167 unrelated HD patients.ResultsThe expanded CAG repeat explained 30.8% of the variance in AO. Adding the genotypes of the SNPs investigated did not affect the variance of the AO variance explained.ConclusionIn this replication study, no association was found explaining a significant amount of the variability in AO of HD thus contradicting a recent report.
Highlights
TP53 is an attractive candidate for modifying age of onset (AO) in Huntington disease (HD): The amino-terminus of the mutated huntingtin exon 1 translation product has functional properties which may affect critically the TP53 pathway in HD neurons
HD is caused by an expansion of a polyglutamine tract in the amino-terminal portion of the protein huntingtin, which apparently acquires a deleterious gain of function [2]
The length of the polyglutamine tract is the most important factor in determining AO of HD, substantial variability remains after controlling for repeat length, in cases where CAG repeat numbers range in the high 30 s or low 40 s [3]
Summary
TP53 is an attractive candidate for modifying age of onset (AO) in Huntington disease (HD): The amino-terminus of the mutated huntingtin (htt) exon 1 translation product has functional properties which may affect critically the TP53 pathway in HD neurons. The pathogenic domain of mutant htt interacts with nuclear transcription factors, and it potentially modulates TP53-induced transcriptional events. HD is caused by an expansion of a polyglutamine tract in the amino-terminal portion of the protein huntingtin (htt), which apparently acquires a deleterious gain of function [2]. The length of the polyglutamine tract is the most important factor in determining AO of HD, substantial variability remains after controlling for repeat length, in cases where CAG repeat numbers range in the high 30 s or low 40 s [3]. A variety of intracellular stress signals activate TP53 to induce either transient cell (page number not for citation purposes)
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