Age and Breeding Effort as Sources of Individual Variability in Oxidative Stress Markers in a Bird Species

Abstract

Oxidative stress is the imbalance between the production of pro-oxidant substances and the level of antioxidant defenses, which leads to oxidative damage. It has been proposed that senescence is the result of accumulated oxidative damage throughout life. In birds, the sources of individual variability in oxidative stress are still poorly understood. Among these sources, age, as related to senescence, should be particularly relevant. Furthermore, recent findings suggest that breeding effort may also deeply influence susceptibility to oxidative stress. However, there is still no evidence of a link between breeding effort and oxidative damage in any vertebrate. Here we analyzed 288 captive red-legged partridges (Alectoris rufa) across a wide age range (i.e., 1-8 yr old), thus including potentially senescent birds. In spite of limitations due to the cross-sectional approach, results revealed that old birds produced less offspring and endured higher levels of oxidized glutathione and peroxidized lipids in erythrocytes than did middle-aged individuals. Old birds also showed higher plasma total antioxidant status and uric acid levels than did younger birds, but lower amounts of circulating carotenoids. Furthermore, hatching success was negatively correlated to lipid peroxidation in females but not in males, supporting the hypothesis that breeding effort promotes oxidative damage.