Agave angustifolia albino plantlets lose stomatal physiology function by changing the development of the stomatal complex due to a molecular disruption.

Abstract

Stomatal development is regulated by signaling pathways that function in multiple cellular programs, including cell fate and cell division. However, recent studies suggest that molecular signals are affected by CO2 concentration, light intensity, and water pressure deficit, thereby modifying distribution patterns and stomatic density and likely other foliar features as well. Here, we show that in addition to lacking chloroplasts, the albino somaclonal variants of Agave angustifolia Haw present an irregular epidermal development and morphological abnormalities of the stomatal complex, affecting the link between the stomatal conductance, transpiration and photosynthesis, as well as the development of the stoma in the upper part of the leaves. In addition, we show that changes in the transcriptional levels of SPEECHLESS (SPCH), TOO MANY MOUTHS (TMM), MITOGEN-ACTIVATED PROTEIN KINASE 4 and 6 (MAPK4 and MAPK6) and FOUR LIPS (FLP), all from the meristematic tissue and leaf, differentially modulate the stomatal function between the green, variegated and albino in vitro plantlets of A. angustifolia. Likewise, we highlight the conservation of microRNAs miR166 and miR824 as part of the regulation of AGAMOUS-LIKE16 (AGL16), recently associated with the control of cell divisions that regulate the development of the stomatal complex. We propose that molecular alterations happening in albino cells formed from the meristematic base can lead to different anomalies during the transition and specification of the stomatal cell state in leaf development of albino plantlets. We conclude that the molecular alterations in the meristematic cells in albino plants might be the main variable associated with stoma distribution in this phenotype.