Abstract

Strains of the entomopathogens Isaria fumosorosea and Metarhizium anisopliae were grown on PdA plates containing chitin azure to determine whether chitinase expression could be correlated with their previously measured pathogenic effects against Formosan subterranean termites. Isaria strains 3581 and 4491 formed chitin azure conversion zones that were significantly larger, 40.0 mm on day 12, than the zone formed by strain 92118, 36.8 mm on day 12. However in past studies, strain 4491 was less pathogenic than strain 92118. the zones formed by M. anisopliae strain 30905 were significantly smaller than those formed by the Isaria strains, 7.0 mm on day 12, but the Metarhizium strain is known to cause mortality of termites. to determine if the glucose in PdA inhibited chitinase expression, the experiment was repeated on A&M plates supplemented with fructose instead of glucose. More variation was seen between strains 3581 and 4491, but the expression of chitinase was not enhanced.

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