Ag-TiO2/biofilm/nitrate interface enhanced visible light-assisted biodegradation of tetracycline: The key role of nitrate as the electron accepter

Abstract

Due to the pivotal role of Ag-TiO2/biofilm/nitrate interface, enhanced visible light-assisted biodegradation of tetracycline (TC) in anoxic system was realized through both batch experiment and long-term operation in this study. The results of the batch experiment elucidated that 50 mg L−1 TC could be completely removed within 10 h in Ag-TiO2/biofilm/nitrate system. The continuous flow experiment was operated for 75 d to evaluate the performance and stability of Ag-TiO2/biofilm/nitrate system. TC removal efficiency in Ag-TiO2/biofilm/nitrate system was as high as 92.4 ± 1.6% at influent TC concentration of 50 mg L−1 TC and hydraulic retention time (HRT) of 10 h, which would be attributed to the promoted separation of photoholes and photoelectrons at the presence of nitrate as electron acceptor. Facilitated electron transfer between semiconductor and biofilm was beneficial for enhancing TC biodegradation, thus lowering toxicity of intermediate products and promoting microbial activity. Moreover, the species related to TC biodegradation (Rhodopseudomonas, Phreatobacter and Stenotrophomonas), denitrification (Thauera) and electron transfer (Delftia) were enriched at Ag-TiO2/biofilm/nitrate interface. Besides, a possible mechanism involved in enhanced TC degradation and nitrogen removal at Ag-TiO2/biofilm/nitrate interface was proposed. This study provided a novel and promising strategy to enhance recalcitrant TC removal from industrial wastewater.