Afterhyperpolarization time-course and minimal discharge rate in low threshold motor units in humans

Abstract

The alpha-motoneurone afterhyperpolarization (AHP) duration correlates with a number of its muscle unit properties in animal preparations. In humans, the interval death rate (IDR) analysis has been used to estimate the time course of human motoneurone AHP based on the pattern of motor unit firing. The purpose of this experiment was first, to examine the relationship between estimated AHP time course and the minimal firing rate of the motor unit and second, to examine the relationship between the AHP and motor unit contractile properties in the tibialis anterior (TA) muscle. Motor unit data were obtained from the TA muscle during low force isometric contractions lasting 600 s. Muscle unit twitch characteristics were determined using spike-triggered averaging (STA) and the motoneurone AHP time course was estimated using the IDR analysis. Minimal discharge rate and derecruitment threshold torque were determined for 2 s preceding motor unit derecruitment. The AHP time constant and minimal discharge rate were negatively correlated, whereas the derecruitment threshold torque was not associated with the AHP time constant. The estimated AHP duration, however, is considerably shorter than the mean ISI of the minimal discharge rate suggesting that synaptic noise and AHP duration are important factors in dictating the minimal discharge rate in low force voluntary contractions in humans. The AHP time constant did not vary significantly with motor unit twitch amplitude; however, significant positive relationships were found between the AHP time constant and the temporal properties of the motor unit twitch. The calculated AHP time course using the IDR analysis, therefore, is a reasonable estimate and coupled with motor unit properties attained with STA, it provides a powerful method to describe low-threshold motor units.