Afterhyperpolarization of human motoneurons firing double and triple discharges.

Abstract

During isometric voluntary contractions of a healthy human muscle, motoneurons (MNs) fire usually with low mean rates, rarely exceeding 25/s (e.g., Garland and Griffin, 1999). However, there are MNs, which sometimes fire double discharges (doublets) with interspike interval (ISI) of few ms. This is observed seldom in normal MNs (e.g., Denslow, 1948; Kudina, 1974; Bawa and Calancie, 1983) but does so more often in neuromuscular disorders (Partanen, 1978; Kostera-Pruszczyk et al., 2002; Piotrkiewicz et al., 2008), where it is considered to be an early sign of MN dysfunction (Rowinska-Marcinska et al., 1999). Our special attention is paid to the so-called “true” doublets recorded in some MNs under conditions of constant synaptic drive, as opposed to those recorded during rapid contractions (Bawa and Calancie, 1983; Kudina and Andreeva, 2013a). Initial doublets, observed during repetitive movements such as locomotion (Zajac and Young, 1980; Hennig and Lomo, 1987) or respiration (Kirkwood and Munson, 1996), where the synaptic drive undergoes periodic changes, also cannot be classified as “true” doublets. For the sake of clarity, the adjective “true” will be omitted in the further text. It has been observed that not each human MN is capable of firing doublets (Kudina, 1974; Bawa and Calancie, 1983). By the analogy to animal studies, it was hypothesized that doublets may be generated only in these MNs, which possess the delayed depolarization (DD, Granit et al., 1963; Kernell, 1964; Calvin, 1974) with a prominent hump that may spontaneously cross the firing threshold and evoke an extra spike (Nelson and Burke, 1967; Calvin, 1973). This hypothesis was verified by Kudina and Churikova (1990), who tested the changes in excitability of human motoneurons within ISI by studying the responses of single MNs to the stimulation of Ia afferents. In MNs capable of firing doublets they revealed the period of increased excitability during the first 15 ms after regular discharge, which corresponded well to the duration of DD observed in animal experiments (Granit et al., 1963; Kernell, 1964; Nelson and Burke, 1967; Calvin, 1973). Nowadays, DD is widely accepted as the mechanism responsible for doublet generation in human MNs (e.g., Kudina, 1974; Bawa and Calancie, 1983; Kudina and Churikova, 1990; Garland and Griffin, 1999). However, it can explain only the origin of occasional doublets, interspersed in regular rhythmic MN activity. Repetitive doublets, i.e., series of doublet-postdoublet ISIs, require some additional mechanism, which would support the DD hump, shown to disappear during MN rhythmic firing (Granit et al., 1963; Calvin and Loeser, 1975). It was recently hypothesized that repetitive doublet firing may be related to plateau potentials (Kudina and Andreeva, 2010, 2013a). It is still under debate, whether DD is related to other characteristics of a MN, measurable in human experiments. Kernell (1964) noted that DD with a definite hump had a tendency to be more common in MNs with short afterhyperpolarization (AHP), although it could occur with an AHP of any duration. Also in humans, MNs capable of firing doublets were more often found in the fast muscles (Bawa and Calancie, 1983; Kudina and Alexeeva, 1992) than in the slower ones (Andreassen and Rosenfalck, 1980; Kudina and Andreeva, 2013b). For the long time it was believed that the MNs supplying one of the most often investigated slow human muscle, the soleus, are devoid of DD. However, it has been recently shown that also MNs supplying human soleus may fire doublets (Piotrkiewicz et al., 2013), which seems to question Kernell's finding. In the present paper we will compare the estimates of AHP duration of MNs capable and not capable of firing double discharges and comment on this controversy. We will also propose a hypothesis on the AHP-related mechanism underlying firing of triplets, which can be observed in some doublet firing MNs.