Abstract
To investigate an outbreak of African horse sickness (AHS) on a horse farm in northeastern Thailand, we used whole-genome sequencing to detect and characterize the virus. The viruses belonged to serotype 1 and contained unique amino acids (95V,166S, 660I in virus capsid protein 2), suggesting a single virus introduction to Thailand.
Highlights
African horse sickness virus (AHSV) is an RNA virus of the family Reoviridae, genus Orbivirus
On April 26, horses on the farm were vaccinated with polyvalent, live-attenuated AHSV vaccine (Ondersterpoort Biological Products, https://www.obpvaccines.co.za); no horses showed clinical signs after vaccination and implementation of insect-proof housing
African Horse Sickness Virus Serotype 1, Thailand samples were tested for antibodies against AHSV by blocking ELISA specific to VP7 (INgezim AHSV Compac Plus; Eurofins Technologies, https://ingenasa.eurofins-technologies.com) (Appendix)
Summary
African horse sickness virus (AHSV) is an RNA virus of the family Reoviridae, genus Orbivirus. On April 26, horses on the farm were vaccinated with polyvalent, live-attenuated AHSV vaccine (Ondersterpoort Biological Products, https://www.obpvaccines.co.za); no horses showed clinical signs after vaccination and implementation of insect-proof housing. African Horse Sickness Virus Serotype 1, Thailand samples were tested for antibodies against AHSV by blocking ELISA specific to VP7 (INgezim AHSV Compac Plus; Eurofins Technologies, https://ingenasa.eurofins-technologies.com) (Appendix). Blood samples from horse CU1; lymph nodes from CU-2; and lymph node, lung, spleen, heart, liver, and kidney samples from CU-3 were positive for AHSV (Appendix Table 4).
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