Abstract
Abstract The amplified fragment length polymorphism (AFLP) assay is an efficient method for the identification of molecularmarkers, useful in the improvement of numerous crop species. Bulked Segregant Analysis (BSA) was used toidentify AFLP markers associated with water-stress tolerance in barley, as this would permit rapid selection ofwater-stress tolerant genotypes in breeding programs. AFLP markers linked to water-stress tolerance was identifiedin two DNA pools (tolerant and sensitive), which were established using selected F 2 individuals resulting from acrossbetween water-stress-tolerant and sensitive barley parental genotypes, based on their paraquat (PQ) tolerance, leafsize, and relative water content (RWC). All these three traits were previously shown to be associated withwater-stress tolerance in segregating F 2 progeny of the barley cross used in a previous study. AFLP analysis wasthen performed on these DNA pools, using 40 primer pairs to detect AFLP fragments that are present/absent,respectively, in the two pools and their parental lines. One separate AFLP fragment, which was present in thetolerant parent and in the tolerant bulk, but absent in the sensitive parent and in the sensitive bulk, was identified.Polymorphism of the AFLP marker was tested among tolerant and sensitive F
Highlights
Water stress is one of the greatest yield-reducing factors
The main objective of this study was to determine whether different polymorphic amplified fragment length polymorphism (AFLP) markers could be identified in barley bulks made up based on traits associated with water-stress tolerance
Since water-stress tolerance is a quantitatively inherited trait, controlled by several genetic loci, which are difficult to measure (Forster et al, 2000), identification of molecular markers associated with a major locus contributing to water-stress tolerance would be useful for indirect selection for water-stress tolerance (Altinkut and Gozukirmizi, 2003)
Summary
Water stress is one of the greatest yield-reducing factors It causes various physiological and biochemical effects on plants (Tanaka et al, 1990; Irigoyen et al, 1992; Smirnoff, 1993; Bohnert and Jensen, 1996; Jamaux, 1997; Tabaeizadeh, 1998), and is a serious problem in many parts of the world where barley, wheat, and other small-grained cereals form the staple diets (Quarrie et al, 1999).
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