AFLP and MSAP Analysis on Genetic Variation in Early Generations of Artificially Synthesized Brassica napus

Abstract

In order to reveal pattern of genetic and epigenetic changes following allopolyploidization of A and C genomes in Brassica,AFLP(amplified fragment length polymorphism) was utilized to detect level of genome changes for S0 plants of cross A(Qinghai Dahuang×Zhonghua Jielan) and S0,S1 plants of cross B(Qinghai Dahuang×Zhongchi Jielan).Moreover,MSAP(me-thylation sensitive amplification polymorphism) was utilized to detect level of methylation changes for S0 plants of cross B.Five hundred and twenty three bands were amplified in S0 plants of cross A with 16 pairs of primer,nine out of these bands amplified showed significant differences between S0 and parents plants,mainly including deletion of seven parental restriction fragments and gain of two new fragments,accounting for 1.33% and 0.38% of total fragments,respectively.One thousand and ninety three bands were amplified in parental plants of B cross with 45 pairs of primer,only one from C genome out of these fragments lost in all S0 plants,accounting for 0.09% of total bands.In 16 S1 plants from the B9 plant,10 out of 1092 bands amplified showed varia-tions,consisting of the deletion of nine fragments from C genome and the gain of one new fragment.By MSAP approach,three loci with cytosine methylation changes were founded on A genome of S0 plants of B cross,which accounted for 1.37% of loci detected.In addition,a plant with flower color mutation in S0 generation of B cross was observed;we speculated that the flower color mutation was probably caused by genetic changes on C genome.This study provided theoretical informations for innovation of rapeseed germplasm by resynthesizing Brassica napus.