Abstract
Aflatoxins are secondary metabolites produced by Aspergillus fungi. Feed exposure to aflatoxins may cause a decreased immune system and low productivity outcomes in poultry. This study conducted a limited survey to determine aflatoxin contaminations in feedstuff. 34 feed samples were analyzed to determine aflatoxin B1, aflatoxin B2, aflatoxin G1, and aflatoxin G2 using High-Performance Liquid Chromatography (HPLC) fluorescent detector. Aflatoxins were extracted from feed samples using acetonitrile : water (84:16,v/v), purified with a solid phase extraction (SPE) column, and detected through a fluorescent detector at wavelengths 365 nm (excitation) and 450 nm (emission). The results show a high concentration of AFB1 contamination in 34 samples. Aflatoxin B1 was found in all samples, ranging from 1.41-42.16 ng/g. Eleven samples were found to contain AFG1 ranging from 4.51-6.39 ng/g. One sample contained AFG2 with a concentration of 7.59 n/g. Also, 16 samples contain AFB2 with a concentration ranging from 1.62-2.3 ng/g. The results above show a high prevalence (100%) of AFB1 contamination. The contamination of aflatoxins especially AFB1 in the feedstuffs were harmful to the animals and should be a concern to prevent any adverse effect on public health.
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