Abstract
ABSTRACTAflatoxin B1 (AFB1) occurrence in peanut oil samples randomly collected from family workshops in western Guangdong during 2016–2017 (n = 427) was surveyed. AFB1 content was screened by enzyme-linked immunosorbent assay (ELISA) protocol and analytically confirmed with an UPLC-MS/MS method. The limit of detection of the ELISA method was 1.08 μg kg−1. The recovery values ranged from 84.4 to 92.6% with relative standard deviations of 2.2 to 4.8%. AFB1 was quantified in 47 samples (22.5%) with a range of 15.4–49.9 μg kg−1 in 2016 and in 33 samples (15.1%) with 8.8–22.2 μg kg−1 in 2017, respectively. The AFB1 contamination in peanut oil was season-depended in western Guangdong with the worst case in spring (24.2–37.9%). Overall, a significant reduction of AFB1 occurrence was observed in western Guangdong after 2016. It is advisable to control AFB1 in bulk and self-pressed oil from family workshops and regulate it mandatorily if necessary.
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