Abstract
A sensitive electrochemical molecularly-imprinted sensor was developed for the detection of aflatoxin B1 (AFB1), by electropolymerization of p-aminothiophenol-functionalized gold nanoparticles in the presence of AFB1 as a template molecule. The extraction of the template leads to the formation of cavities that are able to specifically recognize and bind AFB1 through π-π interactions between AFB1 molecules and aniline moities. The performance of the developed sensor for the detection of AFB1 was investigated by linear sweep voltammetry using a hexacyanoferrate/hexacyanoferrite solution as a redox probe, the electron transfer rate increasing when the concentration of AFB1 increases, due to a p-doping effect. The molecularly-imprinted sensor exhibits a broad linear range, between 3.2 fM and 3.2 µM, and a quantification limit of 3 fM. Compared to the non-imprinted sensor, the imprinting factor was found to be 10. Selectivity studies were also performed towards the binding of other aflatoxins and ochratoxin A, proving good selectivity.
Highlights
Aflatoxin B1 (Figure 1) (CAS No 1162-65-8) whose formal name is 2,3,6aR,9aS-tetrahydro-4methoxy-1H,11H-cyclopenta[c]furo[3',2':4,5]furo[2,3-h][1]benzopyran-1,11-dione is an aflatoxin produced by Aspergillus flavus and A. parasiticus
An electrochemical sensor for the sensitive and selective detection of aflatoxin B1, based on a molecularly-imprinted MOF with recognition sites for aflatoxin B1, is prepared through electropolymerization of p-aminothiophenol (PATP)-functionalized AuNPS in the presence of the AFB1 as a template molecule, combining the advantages of molecular imprinting and electrodeposition with those conferred by MOF
The results show that the proposed biosensor has high selectivity for aflatoxin B1
Summary
Aflatoxin B1 (Figure 1) (CAS No 1162-65-8) whose formal name is 2,3,6aR,9aS-tetrahydro-4methoxy-1H,11H-cyclopenta[c]furo[3',2':4,5]furo[2,3-h][1]benzopyran-1,11-dione is an aflatoxin produced by Aspergillus flavus and A. parasiticus. Immunosensors with molecularly-imprinted polymers are bound to be an alternative to traditional immunosensors based on antibodies This is due to the unique combination of advantages displayed by the artificial materials, including the absence of animal inoculation and sacrifice, unnecessary hapten conjugation to carrier protein for stimulated production, the possibility of manufacturing MIPs against toxic substances, excellent physicochemical stability, reusability, ease of storage, and recognition in organic media [14]. An electrochemical sensor for the sensitive and selective detection of aflatoxin B1, based on a molecularly-imprinted MOF with recognition sites for aflatoxin B1, is prepared through electropolymerization of p-aminothiophenol (PATP)-functionalized AuNPS in the presence of the AFB1 as a template molecule, combining the advantages of molecular imprinting and electrodeposition with those conferred by MOF.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
