Aflatoxin B1 and fumonisin B1 affect the oxidative status of bovine peripheral blood mononuclear cells

Abstract

Mycotoxins are secondary metabolites having a high cytotoxic potential. They are produced by molds and released in food and feed. To date, the mechanisms underlying the mycotoxin-induced cytotoxicity have not been fully clarified. The induction of oxidative stress, as a possible mechanism, has been postulated. This in vitro study was focused on the effect of two widely occurring mycotoxins, aflatoxin B1 (AFB1) and fumonisin B1 (FB1), on the oxidative status of bovine peripheral blood mononuclear cells (PBMC) incubated for 2 and 7days at different levels of AFB1 (0, 5 and 20μg/ml) and FB1 (0, 35 and 70μg/ml). Reactive oxygen metabolites (ROM), intracellular thiols (SH), malondialdehyde (MDA) and gene expression of cytoplasmic superoxide dismutase (SOD) and glutathione peroxidase (GSHPX-1) were measured on PBMC after incubation. The highest concentration of AFB1 and all concentrations of FB1 caused an increase (p<0.05) of intracellular ROM without any time dependent effect. Intracellular SH decreased with 20μgAFB1/ml (p<0.05) and the effect was particularly marked after 7days of exposure. Intracellular SH were not affected by FB1 even though a lower (p<0.05) SH level after 2days exposure than after 7days was observed. MDA increased (p<0.05) in AFB1 or FB1 treated PBMC. The exposure to FB1 for 7days increased MDA (p<0.05) only in cells treated with 70μg/ml. Exposure of PBMC to AFB1 reduced SOD mRNA while FB1 decreased both SOD and GSHPX-1 mRNA abundance. These results demonstrate that, even though by different mechanisms, AFB1 and FB1 may induce cytotoxicity through an impairment of the oxidative status of PBMC.