Abstract
Purified plant cell wall ghosts with high pectin content were investigated for affinity sorption of the galactose specific plant lectins from Ricinus communis L., Glycine max (L.) Merr. and Vviscum album L. The experiments were carried out with the previously described material Permselect prepared from suspension cultured cells of Chenopodium album L. which has been originally developed for permeation chromatography, and a similar material obtained from parenchyma of cabbagehead ( Brassica oleracea L.). After a pretreatment with 2% Na 2CO 3 at room temperature, both cell wall materials showed strongly increased capacity for affinity binding of galactose specific lectins from the crude extracts. Ricin (RCA 2) is the Ricinus lectin preferently bound to pretreated Permselect. Results are discussed with respect to cell wall composition and porosity as well as practical application of Permselect for purification of galactose specific lectins.
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