Abstract
AbstractThe application of a small‐scale method for the preparation of antibodies specific for coat proteins of simple plant viruses is described. The method entails the preparative electrophoresis of crude virion extracts, electrophoretic transfer of the resolved proteins onto nitrocellulose paper, excision of a specific protein band, and the attachment and subsequent elution of antibodies specific for the excised protein. The method was tested with brome mosaic virus (BMV) and low molecular weight plantproteins. It was possible to produce antibodies from multiply‐reactive sera that reacted only with the proteins used to purify them. Antibodies eluted from BMV coat protein monomer reacted strongly with intact virus in indirect ELISA. Other possible applications of the technique to plant virology are discussed.
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