Affinity of Serum Albumin and Fibrinogen to Cellulose, Its Hydrophobic Derivatives and Blends.

Rupert Kargl,Tamilselvan Mohan,Miran Mozetič,Wolfgang Bauer,Matej Bračič,Matic Resnik,Karin Stana Kleinschek

doi:10.3389/fchem.2019.00581

Rupert Kargl, Tamilselvan Mohan + Show 5 more

Open Access

https://doi.org/10.3389/fchem.2019.00581

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Abstract

This work describes the preparation of spin-coated thin polymer films composed of cellulose (CE), ethyl cellulose (EC), and cellulose acetate (CA) in the form of bi- or mono-component coatings on sensors of a quartz crystal microbalance with dissipation monitoring (QCM-D). Depending on the composition and derivative, hydrophilicity can be varied resulting in materials with different surface properties. The surfaces of mono- and bi-component films were also analyzed by atomic force microscopy (AFM) and large differences in the morphologies were found comprising nano- to micrometer sized pores. Extended protein adsorption studies were performed by a QCM-D with 0.1 and 10 mg mL−1 bovine serum albumin (BSA) and 0.1 and 1 mg mL−1 fibrinogen from bovine plasma in phosphate buffered saline. Analysis of the mass of bound proteins was conducted by applying the Voigt model and a comparison was made with the Sauerbrey wet mass of the proteins for all films. The amount of deposited proteins could be influenced by the composition of the films. It is proposed that the observed effects can be exploited in biomaterial science and that they can be used to extent the applicability of bio-based polymer thin films composed of commercial cellulose derivatives.

Highlights

Synthetic and bio-based polymeric biomaterials are widely used for biomedical devices including those which are in contact with human blood, plasma, serum, or protein solutions
This work aims at investigating and comparing the interaction of the proteins bovine serum albumin (BSA) and fibrinogen (FIB) on commercially available celluloses and their derivatives in the form mono- and bicomponent thin films using a quartz crystal microbalance with dissipation monitoring (QCM-D). Both proteins are essential in the assessment of biocompatibility and blood coagulation and any material composed of these polymers that comes into contact with full blood or plasma will be initially covered by these proteins as confirmed by QCM-D and surface plasmon resonance (SPR) (Mohan et al, 2013, 2014, 2017)
Care must be taken that the surface morphology and accessibility of the films for water and protein are considered with respect to the QCM-D and contact angle results

Summary

Introduction

Synthetic and bio-based polymeric biomaterials are widely used for biomedical devices including those which are in contact with human blood, plasma, serum, or protein solutions. This work aims at investigating and comparing the interaction of the proteins bovine serum albumin (BSA) and fibrinogen (FIB) on commercially available celluloses and their derivatives in the form mono- and bicomponent thin films using a quartz crystal microbalance with dissipation monitoring (QCM-D) Both proteins are essential in the assessment of biocompatibility and blood coagulation and any material composed of these polymers that comes into contact with full blood or plasma will be initially covered by these proteins as confirmed by QCM-D and surface plasmon resonance (SPR) (Mohan et al, 2013, 2014, 2017). Understanding protein adsorption on mono- and bicomponent films of cellulose and its derivatives should allow for a basic understanding of their surface properties and result in alternative applications for these bio-based polymers

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